17beta-Estradiol ELISA

Enzyme immunoassay for the quantitative determination of estradiol in human serum.

INTENDED USE:For the quantitative measurement of estradiol in human serum by an ELISA (enzyme-linked immunosorbent assay). This kit is intended for professional use only and is for laboratory use only. For in vitro diagnostic use only. Intended to be used manually but may be adaptable to open automated analyzers. The user is responsible for validating the performance of this kit with any automated analyzers.

GENERAL INFORMATION:17β-Estradiol, or Estradiol, is predominantly produced by the ovaries in premenopausal females, testes in males, and in adipose tissue and the adrenal glands of males and females. Males and postmenopausal females have the lowest estradiol concentration levels. In non-pregnant, premenopausal females, estradiol concentrations fluctuate during the menstrual cycle with a peak just before ovulation. Estradiol remains elevated during the luteal phase, before decreasing to lower concentrations during the follicular phase.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 1 h, 30 min (RT/shaker)
- Standard Range: 25 - 4,000 pg/ml
- Sensitivity analytical: LoD: 8.4 pg/ml
- Final Sample Volume: 50 µl
- Sample Type: serum
- Isotope / Substrate: TMB 450 nm
- Internal Controls 2
- Regulatory Status: CE