Adiponectin ELISA

Enzyme immunoassay for the quantitative determination of Adiponectin in human serum and plasma.

INTENDED USE:For the quantitative determination of Adiponectin in human serum and plasma by an enzyme immunoassay method. For in vitro use only.

GENERAL INFORMATION:Adiponectin is a hormone that modulates glucose regulation and fatty acid oxidation. It is secreted from adipose tissue and placenta into the bloodstream and represents 0.01% of all lasma protein. Adiponectin increases insulin sensitivity and decreases plasma glucose by increasing tissue fat oxidation. Adiponectin concentrations correlate negatively with glucose, insulin and triglycerides (TG) concentrations, liver fat content and body mass index and positively with high density lipoprotein cholesterol levels, hepatic insulin sensitivity and insulin stimulated glucose disposal. Adiponectin levels decrease in patients with type 2 diabetes and in patients with coronary heart disease. Adiponectin could be used as a marker for:
- Energy metabolism and body weight regulation.
- Metabolic syndrome.
- Type 2 diabetes.
- Coronary artery disease.
- Atherosclerosis.

KIT CHARACTERISTICS:
- Method: ELISA
- Tests: 96
- Incubation Time / Conditions: 1 h (RT/shaker), 30 min (RT/shaker), 10-15 min (RT/shaker)
- Standard Range: 2 - 50 ng/ml
- Sensitivity analytical: LoD: 0.06 ng/ml
- Final Sample Volume: 50 µl
- Sample Type: serum, plasma
- Sample Pretreatment: dilution 1:1000
-Isotope / Substrate: TMB 450 nm
- Internal Controls: 2
- Regulatory Status: CE