BactoReal® Kit Pasteurella multocida

Detection of the 16S rRNA gene of Pasteurella multocida (subspecies Pasteurella multocida, Pasteurella gallicida, Pasteurella septica, Pasteurella tigris)

Background: The Gramnegative bacterium Pasteurella multocida is able to cause disease in a wide range of animal species and in humans. Pasteurella multocida can cause fowl cholera disease in poultry, hemorrhagic septicemia and shipping fever in cattle, atrophic rhinitis in pigs and is the most common cause of respiratory disease, primarily rhinitis, in rabbits. Although Pasteurella multocida is part of the flora in the respiratory tract of pigs, the bacterium can induce hemorrhagic septicemia or, usually as a secondary pathogen invading lungs injured by other bacteria or viruses, pneumonia. A subset of Pasteurella multocida isolates are critical agents in an upper respiratory disease, progressive atrophic rhinitis (PAR) in pigs.

Description: BactoReal® Kit Pasteurella multocida uses real-time PCR for the detection of the 16S rRNA gene of P. multocida (subsp. multocida, gallicida, septica and tigris). Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: 16S rRNA gene of Pasteurella multocida
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX