BactoReal® Kit Salmonella enterica

Detection of the invA gene of Salmonella enterica

Background: Salmonella is a genus of Gramnegative bacteria of the Enterobacteriaceae family. This genus consists of only two species, Salmonella enterica and Salmonella bongori. Salmonella bongori mostly infects cold-blooded animals. Salmonella enterica has 6 subspecies and more than 2500 serovars. Subspecies are S. enterica subsp. arizonae, S. enterica subsp. diarizonae, S. enterica subsp. enterica, S. enterica subsp. houtenae, S. enterica subsp. indica and S. enterica subsp. salamae. Salmonella infections in warm-blooded animals are generally caused by strains from S. enterica subspecies enterica. Nontyphoidal serovars are more common, and usually cause self-limiting gastrointestinal disease. They can infect a range of animals, and are zoonotic. Although many other Salmonella serovars may cause enteric disease, the more common ones in cattle are Salmonella Typhimurium, Salmonella Dublin and Salmonella Newport. In sheep and goat the most common are Salmonella Typhimurium, Salmonella Dublin, Salmonella Abortusovis, Salmonella Anatum and Salmonella Montevideo. In pigs the most common are Salmonella Typhimurium and Salmonella Choleraesuis, and in poultry Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum and Salmonella Pullorum.

Description: BactoReal® Kit Salmonella enterica uses real-time PCR for the detection of the invasion protein A (invA) gene of Salmonella enterica. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: invA gene of Salmonella enterica
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX