BactoReal® Kit Staphylococcus aureus

Detection of the nuc gene of Staphylococcus aureus

Background: Staphylococcus aureus is a Grampositive bacterium. In healthy animals, S. aureus colonization of the skin, intestinal tract, or nasopharynx is generally asymptomatic. However, depending on the strain, S. aureus is capable of secreting several exotoxins. Many of these toxins are associated with specific diseases. In cattle, S. aureus causes one of the most common types of chronic mastitis.

Description: BactoReal® Kit Staphylococcus aureus uses real-time PCR for the detection of the thermonuclease (nuc) gene of S. aureus. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: nuc gene of Staphylococcus aureus
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX