BactoReal® Kit Staphylococcus spp.

Detection of the 23S rRNA gene of Staphylococcus species

Background: The genus Staphylococcus includes more than 50 different species. Staphylococci are commensals on skin and upper respiratory tracts of mammals and birds. However, they can also cause various infections of the skin and a wide variety of diseases in humans and animals through either toxin production or penetration. Another common path of infection is the respiratory tract, where the bacterium causes pneumonia. Staphylococci, mainly Staphylococcus aureus can also cause bovine mastitis.

Description: BactoReal® Kit Staphylococcus spp. uses real-time PCR for the detection of the 23S rRNA gene of species of the genus Staphylococcus. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: 23S rRNA gene of Staphylococcus spp.
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX