Corticosterone rat/mouse ELISA

Background:Corticosterone is secreted by the adrenal cortex under control of the pituitary hormone ACTH via a negative feedback mechanism. It is the most abundant circulating steroid in rats, since rodents are not able to synthesize Cortisol, the major glucocorticoid in human, as a result of lacking the enzyme C17-Hydroxylase. Corticosterone has a wide range of activities in rodents. It regulates carbohydrate, protein and fat metabolism. It has also an influence on the hemopoietic system and reduces the total number of lymphocytes and eosinophils, but to a lesser extent than cortisol. In contrast to cortisol, corticosterone has only minimal anti-inflammatory activity.

Description:
The Corticosterone rat/mouse ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. An unknown amount of corticosterone present in the sample and a defined amount of corticosterone conjugated to horseradish peroxidase compete for the binding sites of corticosterone antiserum coated to the wells of a microplate. After incubation on a shaker the microplate is washed four times. After addition of the substrate solution the concentration of corticosterone is inversely proportional to the optical density measured.

Product features:
The kit contains reagents for 96 determinations;
Microtiter plate consisting of 12x8 (break-apart) strips;
Microtiter plate reader at 450 nm;
Analytical sensitivity: 6,1 ng/mL.