Real-time PCR for the identification of E. coli O157:H7. AOAC-PTM (#22002), NF VALIDATION (EGS 38/06-11/19). The BACGene E. coli O157:H7 kit is a multiplex real-time PCR kit for the detection and identification of O157:H7 with the option of reporting O157 (excluding H7) and O157:H7 specifically. In combination with the BACGene Mplex SEROtype kits this assay enables the detection of all of the Top7 STEC.