Human Big Endothelin-1 Assay Kit

Background: Endothelins (ETs) are isopeptides produced by vascular endothelium having potent vasoconstriction activity. The peptides are encoded by three separate genes and processed to yield 39 residue Big Endothelin (Big ET) molecule, which are further processed to the 21 amino acid sequences termed Endothelin-1 (ET-1), Endothelin-2 (ET-2) and Endothelin-3 (ET-3). All of members of the endothelin family contain two essential disulfide bridges and size conserved amino acid residues at the C-terminus. The ETs are produced by a variety of issues in vivo, including lung, kidney, brain, pituitary and placenta.

Description:This kit is a solid phase sandwich ELISA using 2 kinds of high specific antibodies. Tetra Methyl Benzidine (TMB) is used as coloring agent (Chromogen). The strength of coloring is in proportion to the quantities of Human Big ET-1.

Product features:
The kit contains reagents for 96 determinations;
Microtiter plate consisting of 12x8;
Microtiter plate reader at 450 nm;
Analytical sensitivity: 0,3 pg/mL