MycoReal Kit Nosema apis & ceranae

Detection of the 18S rRNA gene of Nosema apis and Nosema ceranae

Background: Nosema apis and Nosema ceranae are microsporidian parasites recently reclassified as a fungus. Both species cause nosema disease in honeybees (Apis mellifera). The dormant stages are long-lived spores. The most notable symptom is dysentery. Nosema ceranae can be detected in all four seasons, while Nosema apis occurs mostly in the milder seasons of autumn and spring. There is a higher mortality of bees when they are infected by Nosema ceranae than when infected with Nosema apis. Double infection is also possible.

Description: MycoReal Kit Nosema apis & ceranae uses multiplex real-time PCR for the detection and differentiation of the 18S rRNA gene of Nosema apis as well as of the 18S rRNA gene of Nosema ceranae. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: 18S rRNA of Nosema apis and 18S rRNA of Nosema ceranae
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM & VIC/HEX ; Channel IPC: Cy5