Real Time PCR Detection Kit Panel Cyanotoxin Genes

The Real Time PCR detection Kit Panel Cyanotoxin Genes (Microcystine toxin genes mycB and mycE) comprises a series of specific targeted reagents designed for Microcystine toxin genes mcyB and mcyE detection by using qPCR.

GENERAL INFORMATION:Microcystins (or cyanoginosins) are a class of toxins produced by certain freshwater cyanobacteria, commonly known as blue-green algae. The main productor are species from the Microcystis genus, but other genera as Planktothrix, Anabaena, Nostoc and Fisherella can produce toxins as well. Mycrocystins can cause liver damage to several animal species including dogs, fish, birds, and livestock. Genes involved in the biosynthesis of the toxin are organized into two operons (mcyA-C and mcyD-J). The assay was designed to recognise genes mcyE and mcyB, one from each operon, with a wide range of inclusiveness despite the high degree of diversity achieved for each genera. The presence of this genes in a water sample can be correlated in most cases with the presence of microcystins.

KIT CHARACTERISTICS:
- Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
- Resuspension buffer
- DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
- Positive control