soluble (Pro)renin Receptor ELISA

Background: (Pro)renin receptor/(P)RR is a common receptor protein between renin and prorenin. When prorenin binds to (P)RR, it achieves a binding ability to angiotensinogen and gains a catalytic activity for conversion from angiotensinogen to ansiotensin I in comparable level with renin. Additionally, when the (P)RR is stimulated by binding to prorenin, its intracellular signaling progresses. Through this, (P)RR research is believed to have an important implication in building of new strategy for suppressing of overactivated tissue RAA (Renin-Angiotensin-Aldosterone) system. (P)RR is a 39 kDa, single transmembrane receptor protein and it is reported that 29 kDa soluble form is generated by cleavage of (P)RR with furin. Thus the quantitative assay of soluble (P)RR in blood or urine samples is expected to provide new knowledges to clarification of disease mechanisms and development of new tools for diseases. This ELISA kit can measure concentration of soluble (pro)renin receptor in blood or urein samples. ＊This ELISA kit can also measure full-length (P)RR as well. Therefore, in the case of samples like tissue extract or lysate, this ELISA would measure total (P)RR.

Description:
This kit is a solid phase sandwich ELISA using 2 kinds of highly specific antibodies. Tetra Methyl Benzidine (TMB) is used as a coloring agent (Chromogen). The strength of coloring is proportional to the quantities of (Pro)renin Receptor.

Product features:
The kit contains reagents for 96 determinations;
Microtiter plate consisting of 12x8;
Microtiter plate reader at 450 nm;
Analytical sensitivity: 24 pg/mL