Tenascin-C Large (FNIII-B) ELISA

Background: Tenascin-C is an extracellular matrix (ECM) glycoprotein that is composed of 210-400 kDa subunits consisting of four domains. One subunit has a TA domain at the Nterminal end, then an epidermal growth factor-like sequence domain (EGF-like domain), a fibronectin typeⅢ(FNⅢ) repeat domain, and a fibrinogen-like domain at the C-terminal end. There is an alternatively spliced domain in the FNⅢ domain, and it generates some types of variants of Tenascin-C. The subunits form a trimer by twisting at the N-terminal coiled domain and form a hexamer by a disulfide bond, in tissue. While low molecular weight variants of Tenascin-C are present in normal tissue, it is said that high molecular variants of Tenascen-C is expressed in various diseased tissue including cancer. This kit can detect the Tenascin-C high molecular weight variant including the subunit in which FN Ⅲ -B domain specifically, by using two different kinds of specific antibodies.

Description:
This kit is a solid phase sandwich ELISA using 2 kinds of highly specific antibodies. Tetra Methyl Benzidine (TMB) is used as a coloring agent (Chromogen). The strength of coloring is proportional to the quantity of Tenascin-C High Molecular Weight Variants including FNⅢ-B domain.

Product features:
The kit contains reagents for 96 determinations;
Microtiter plate consisting of 12x8;
Microtiter plate reader at 450 nm;
Analytical sensitivity: 44 pg/mL