ViroReal® Kit Bluetongue Virus (BTV)

Detection of RNA of the segment 10 (NS3) of BTV

Background: Bluetongue virus (BTV) is a segmented, linear, double-stranded RNA virus (dsRNA), belonging to the Reoviridae family and is divided into 28 serotypes. The virus is transmitted by biting midges and infects wild and domestic ruminants such as sheep and less frequently goats, deer and cattle. There is no appropriate treatment of acute disease, which is associated with high morbidity and mortality in some sheep breeds. Prophylactic immunisation with attenuated and inactivated vaccines is available. However, they do not cover all virulent BTV serotypes.

Description: ViroReal® Kit Bluetongue Virus (BTV) uses one-step reverse transcription RT real-time PCR for the detection of the segment 10 (NS3) of BTV. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.

Product Features:
- Amplification and detection: NS3 gene of Bluetongue Virus (BTV)
- Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
- Contains RNase Inhibitor to block RNA degradation
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX
*400 reactions also available (ref: DVEV02213x4)