ViroReal® Kit MERS-CoV

Detection of RNA of the N gene of Middle East Respiratory Syndrome Coronavirus

Background: Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is a beta coronavirus causing Middle East Respiratory Syndrome (MERS), a zoonotic and severe respiratory disease in humans. To date, MERS-CoV has only been isolated from dromedary camels and humans. In humans, it was first identified in April 2012. Camels are a likely primary source of the MERS-CoV infection in humans.

Description: ViroReal® Kit MERS-CoV uses one-step reverse transcription RT real-time PCR for the detection of the nucleocapsid (N) gene of Middle East Respiratory Syndrome Coronavirus (MERS-CoV). Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.

Product Features:
- Amplification and detection: N gene of MERS-CoV
- Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
- Contains RNase Inhibitor to block RNA degradation
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: VIC/HEX; Channel IPC: Cy5