ViroReal® Kit PEDV & SDCV

Detection and differentiation of RNA of the N gene of porcine epidemic diarrhea virus and of the 3’UTR of swine delta coronavirus (PDCoV)

Background: Porcine epidemic diarrhea virus (PEDV), a member of the genus Coronavirus (Alphacoronavirus), family Coronaviridae, is a positive-sense, enveloped, single-stranded RNA virus. Severity of disease is variable and dependent on the epidemiological status of the herd. When epidemic, PEDV causes acute watery diarrhea and vomiting in swine of all ages. If endemic, then diarrhea is observed with lower morbidity in suckling and recently weaned pigs. The PED virus is similar to, but antigenically distinct from transmissible gastroenteritis virus (TGEV).

Description: ViroReal® Kit PEDV & SDCV uses one-step reverse transcription multiplex RT real-time PCR for the detection of nucleocapsid protein gene (N gene) of PEDV (porcine epidemic diarrhea virus) and of the 3’UTR of SDCV (swine delta coronavirus, PDCoV). Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.

Product Features:
- Amplification and detection: N gene of PEDV and 3’UTR of SDCV
- Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
- Contains RNase Inhibitor to block RNA degradation
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM & VIC/HEX; Channel IPC: Cy5