ViroReal® Kit PEDV & TGEV

Detection and differentiation of RNA of the N gene of porcine epidemic diarrhea virus and of the S region of transmissible gastroenteritis virus

Background: The family Coronaviridae includes pathogenic porcine coronaviruses such as the transmissible gastroenteritis virus (TGEV), the porcine respiratory coronavirus (PRCV), the porcine epidemic diarrhea virus (PEDV) and the porcine hemmaglutinating encephalomyelitis virus (PHEV). TGEV causes transmissible gastroenteritis (diarrhea and vomiting) in pigs, mortality is highest in neonates. PRCV is a mutant of TGEV and does not appear to be an important primary pathogen. PEDV is similar to, but antigenically distinct from TGEV. When epidemic, PEDV causes acute watery diarrhea and vomiting in a large proportion of all ages of swine. If PEDV is endemic, then diarrhea is observed with lower morbidity in suckling and recently weaned pigs.

Description: ViroReal® Kit PEDV & TGEV uses one-step reverse transcription multiplex RT real-time PCR for the detection of the nucleocapsid protein gene (N gene) of PEDV as well as a part of the S region of TGEV. The test clearly differentiates between these two viruses and does not detect PRCV that has a large in-frame deletion in the 5’ end of the S gene. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.

Product Features:
- Amplification and detection: N gene of PEDV & TGEV
- Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
- Contains RNase Inhibitor to block RNA degradation
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM & VIC/HEX; Channel IPC: Cy5