ViroReal® Kit RHDV - rabbit haemorrhagic disease virus

Detection of RNA of the vp60 gene of rabbit haemorrhagic disease virus 1&2

Background: Rabbit haemorrhagic disease virus (RHDV), also called rabbit calicivirus (RCV) or european brown hare virus is a nonenveloped, positive-sense, single-stranded RNA calicivirus of the genus Lagovirus that causes rabbit haemorrhagic disease (RHD). This disease was first reported in China in 1984 and is a highly contagious and fatal disease for European rabbit (Oryctolagus cuniculus). There exist three distinct groups: the classic RHDV with the genogroups G1–G5 isolated from 1984 onwards, the antigenic variant RHDVa/G6 identified in 1996, and RHDV2 identified in 2010.

Description: ViroReal® Kit RHDV uses one-step reverse transcription RT real-time PCR for the detection of the capsid protein gene vp60 of rabbit haemorrhagic disease virus (RHDV). Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.

Product Features:
- Amplification and detection: vp60 gene of RHDV
- Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
- Contains RNase Inhibitor to block RNA degradation
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Kits format: 100 reactions or 50 reactions
- Channel Target: FAM; Channel IPC: VIC/HEX