ViroReal® Kit Acute Bee Paralysis Virus

Detection of the RNA of the capsid protein gene of Acute bee paralysis virus

Background:Acute bee paralysis virus (ABPV) is a positive single-stranded RNA virus within the family Dicistroviridae and the genus Aparavirus. It is closely related to to Kashmir bee virus (KBV) and Israeli acute paralysis virus (IAPV), hence all three are summarized in the so-called AKI-complex. Members of that complex play a role in the sudden collapses of honeybee hives and colonies and are responsible for severe economical losses worldwide. The symptoms of ABPV infections are pompous abdomens, trembling wings, flightless bees and lead to sudden death.
To distinguish between the Aparavirus species within the AKI-complex, ingenetix offers individual real-time PCR Kits for detection of Acute bee paralysis virus, Kashmir bee virus and Israeli acute paralysis virus. Moreover a real-time PCR Kit for the universal detection of Aparaviruses is available.

Description:ViroReal® Kit Acute Bee Paralysis Virus is a real-time PCR for the qualitative detection of the RNA of the capsid protein gene of Acute bee paralysis virus. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

Product Features:
- Amplification and detection: RNA of capsid protein gene of Acute Bee Paralysis Virus
- Transcription with thermostable MMLV Reverse Transcriptase
- Contains RNase Inhibitor to block RNA degradation
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR platforms: runs on all established standard real-time PCR platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix)