Zymolyase ® 20T (20000U/g)

Prepared from Arthrobacter luteus and supplied as an ammonium sulfate precipitate of a complex of enzymes, allowing for the production of protoplasts or spheroplasts of various yeast strains.

PRODUCT: Zymolyase® 20T from GRiSP is prepared from Arthrobacter luteus, and is supplied as an ammonium sulfate precipitate of a complex of enzymes. The strong lytic activity against living yeast cell walls is mainly due to the activity of ß-1,3-glucan laminaripentaohydrolase. This enzyme hydrolyzes linear glucose polymers with β-1,3-linkages and releases specifically laminaripentaose as the main and minimum product unit, allowing for the production of protoplasts or spheroplasts of various yeast strains.
Lytic activity varies depending on yeast strain, growth stage of yeast, or cultural conditions. Under the conditions mentioned below, the lytic activity of Zymolyase® 20T is 20.000 U/g,
Application: Preparation of spheroplasts or protoplasts from a variety of yeast strains.