Kit de deteção de PCR em tempo real Clostridium novyi Toxina alfa

O kit de deteção de PCR em tempo real para a toxina alfa de Clostridium novyi compreende uma série de reagentes específicos concebidos para a deteção da toxina alfa de Clostridium novyi utilizando qPCR.

GENERAL INFORMATION:Clostridium novyi is a Gram-positive, endospore-forming, obligate anaerobic bacteria of the Clostridiaceae family. It is ubiquitous, being commonly found in soil and faeces and is pathogenic, causing a wide variety of diseases in man and animals. C. novyi is classified as type A, B, and a non-pathogenic type C; distinguished by the range of toxins they produce (alpha, beta, gamma, delta, epsilon and zeta). When grown in culture, life cycle goes through 3 stages: initial growth wherein no toxin is produced; vigorous growth wherein toxin is produced and spore formation wherein endospores are formed and toxin production decreases. The alpha-toxin is lethal, causes necrosis in cells and is produced on A and B types.

KIT CHARACTERISTICS:
- Assay Mix Target composed by singleplex mixtures of specific forward/reverse primers and probe.
- Resuspension buffer
- DNase/RNase free water
- (OPTIONAL) Internal Control Assay Mix
- Mastermix solution
- Positive control